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Wound healing assay Isolated PSCs were cultured in 6-well plates for 72 h

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Ghrelin peptide exists in one of two forms: unacetylated (desacyl-ghrelin) or acylated at Ser 3 with an octanoyl fatty acid (acyl-ghrelin) via a reaction catalyzed by the endoplasmic reticulum-associated enzyme, ghrelin-O-acyltransferase (GOAT) [16]