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Both 5OXP and OPA are likely general biomarkers for oxidative stress and GSH deficiency rather than APAP specific biomarkers, but elevated urinary 5OXP is seen in humans suffering from APAP overdose and is often clinically reported as anion gap metabolic acidosis.32–34 A systems biology approach, developed using in vitro studies in THLE-2E1 cells (CYP2E1-transfected SV40 large T-antigen immortalized human liver epithelial cells) resulted in a computational model that used the relative concentrations of OPA and 5OXP to predict intracellular concentrations of GSH (Fig