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General Protocol Treated with H 2 O 2 Treated with Antimycin ＜Imaging Conditions＞(Confocal microscopy) Intracellular ROS: Ex = 488, Em = 490-520 nm MitoBright ROS: Ex = 633 nm, Em = 640-700 nm Scale bar: 10 μm Experimental Example: Time lapse imaging of ROS in LPS-treated macrophages In ROS Assay Kit -Photo-oxidation Resistant DCFH-DA- stained RAW264.7 cells, after LPS (Lipopolysaccharide)-treated, the intracellular ROS level was simultaneously observed with a fluorescence microscope

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