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To induce acute liver injury, 8-week-old NSI mice received two consecutive intraperitoneal injections of DMN (7 mg/kg, Sigma, 1.0% dissolved in saline)

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It is likely that a dysbiosis arises in these conditions in which a decrease of beneficial bacteria and their metabolic byproducts occurs along with an increase in detrimental microbial populations and their toxic metabolites, leading to an altered luminal environment [106]