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The H 2 S-dependent BP-reducing effect is thought to be primarily mediated through sulfhydration of ATP-sensitive potassium (K ATP ) channels, which in turn leads to voltage-sensitive channel opening and relaxation of vascular smooth muscle cells.98 However, other potassium channels may also be affected by H 2 S, and additional mechanisms have been suggested in determining the opening/closing of K + channels, including nitrosylation, and a possible cooperation between H 2 S and NO.98 While the relationship between NO and H 2 S in controlling vascular relaxation is still unclear (eg, both upregulation and inhibition of eNOS by H 2 S have been reported),99 there is convincing evidence that H 2 S shares at least some of the vasorelaxing signaling role with NO and H 2 S deficiency and therefore can contribute to vascular dysfunction including hypertension.84,93,94,100,101 Nonenzymatic conversion of garlic-derived organic polysulfides to H 2 S In a series of elegant experiments, Benavides et al69 showed that garlic-derived polysulfides can produce H 2 S under physiologically relevant O 2 conditions in rat aortic tissue
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