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Absorbance measurements were made at 405 nm for 12 min at 1 min intervals with a microplate reader using kinetic mode (Cytation 3 multi-mode, BioTek)

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Cartilage matrix is degraded by connective cathepsin released by hypertrophic synovial tissue, chondrocytes and intravascular tissue, which will eventually lead to the destruction of articular cartilage and bone (55, 56)