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Cell Culture and Cytotoxicity Studies Immortal human colon epithelial cells, Caco-2, were grown in Dulbecco’s Modified Eagle’s Medium (DMEM, Corning/Cellgro, VA) supplemented with 10% fetal bovine serum (GIBCO, Life Technologies, NY) and 1X penicillin/streptomycin (Corning/Cellgro, VA)

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These results highlight the diverse characteristics of the population in relation to serum vitamin B12 levels