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For example, glutamine is used to make molecules called purines

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Determination of mucosal level of nitric oxide Gastric mucosa was filtered by Amicon ultra centrifugal filter units 30 kDa (Sigma Chemical Co., MO, USA) then 40 μl of the filtrate was used for determination of NO content by measuring its stable metabolites nitrite (NO 2 ) and nitrate (NO 3 ) using a commercially available ELISA kit (Cayman, Ann Arbor, MI, USA)