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Avoid mixing copper peptides with strong, low‑pH actives like high‑strength vitamin C (ascorbic acid), aggressive AHAs/BHAs (glycolic, lactic, salicylic acid), and potent retinoids in the same step, as they can destabilize peptides or increase irritation

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These sequences were then run through an R script (Supplementary Materials, bash_DADA2 + IDTAXA_workflow_Illumina-PairedEnd_V1V3.R) which trimmed reverse reads to 260 bp, removed forward reads exceeding a maximum expected error of three, and truncated reverse reads exceeding a maximum expected error of six